Similarly to serial X-ray crystallography, this approach leads to almost complete absence of radiation damage effects even for the most sensitive samples, and allows for a high level of automation. Serial electron diffraction (SerialED) is an emerging technique, which applies the snapshot data-collection mode of serial X-ray crystallography to three-dimensional electron diffraction (3D Electron Diffraction), forgoing the conventional rotation method. 4Department of Chemistry, University of Toronto, Toronto, ON, Canada.3Department of Physics, University of Toronto, Toronto, ON, Canada.2Centre for Structural Systems Biology, Department of Chemistry, University of Hamburg, Hamburg, Germany.1Max Planck Institute for the Structure and Dynamics of Matter, Center for Free-Electron Laser Science, Hamburg, Germany.Robert Bücker 1,2* †, Pascal Hogan-Lamarre 1,3,4 † and R.